Commission Regulation (EC) No 271/2009 of 2 April 2009 concerning the authorisation of a preparation of endo-1,4-beta-xylanase and endo-1,4-beta-glucanase as a feed additive for weaned piglets, chickens for fattening, laying hens, turkeys for fattening and ducks for fattening (holder of the authorisation BASF SE) (Text with EEA relevance)
Modified by
  • Commission Implementing Regulation (EU) No 1070/2014of 10 October 2014amending Regulation (EC) No 271/2009 as regards the minimum content of the preparation of endo-1,4-beta-xylanase produced by Aspergillus niger (CBS 109.713) and endo-1,4-beta-glucanase produced by Aspergillus niger (DSM 18404) as a feed additive for laying hens (holder of authorisation BASF SE)(Text with EEA relevance), 32014R1070, October 11, 2014
  • Commission Implementing Regulation (EU) 2021/981of 17 June 2021concerning the renewal of the authorisation of a preparation of endo-1,4-beta-xylanase produced by Aspergillus niger CBS 109.713 and endo-1,4-beta-glucanase produced by Aspergillus niger DSM 18404 as a feed additive for poultry species, ornamental birds and weaned piglets (holder of the authorisation: BASF SE), and repealing Regulation (EC) No 271/2009 and Implementing Regulation (EU) No 1068/2011(Text with EEA relevance), 32021R0981, June 18, 2021
Corrected by
  • Corrigendum to Commission Regulation (EC) No 271/2009 of 2 April 2009 concerning the authorisation of a preparation of endo-1,4-beta-xylanase and endo-1,4-beta-glucanase as a feed additive for weaned piglets, chickens for fattening, laying hens, turkeys for fattening and ducks for fattening (holder of the authorisation BASF SE), 32009R0271R(01), April 8, 2009
Commission Regulation (EC) No 271/2009of 2 April 2009concerning the authorisation of a preparation of endo-1,4-beta-xylanase and endo-1,4-beta-glucanase as a feed additive for weaned piglets, chickens for fattening, laying hens, turkeys for fattening and ducks for fattening (holder of the authorisation BASF SE)(Text with EEA relevance)
Article 1The preparation specified in the Annex, belonging to the additive category "zootechnical additives" and to the functional group "digestibility enhancers", is authorised as an additive in animal nutrition subject to the conditions laid down in that Annex.
Article 2This Regulation shall enter into force on the 20th day following its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.ANNEX
1 TXU is the amount of enzyme which liberates 5 micromoles of reducing sugars (xylose equivalents) from wheat arabino-xylan per minute at pH 3,5 and 40 °C.1 TGU is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley ß-glucan per minute at pH 3,5 and 40 °C.Details of the analytical methods are available at the following address of the Community Reference Laboratory: www.irmm.jrc.be/crl-feed-additivesCategory of zootechnical additives. Functional group: digestibility enhancers.
Identification number of the additiveName of the holder of authorisationAdditiveComposition, chemical formula, description, analytical methodSpecies or category of animalMaximum ageMinimum contentMaximum contentOther provisionsEnd of period of authorisation
Units of activity/kg of complete feedingstuff with a moisture content of 12 %
4a7BASF SEEndo-1,4-beta-xylanaseEC 3.2.1.8Endo-1,4-beta-glucanaseEC 3.2.1.4Additive composition:Preparation of endo-1,4-beta-xylanase produced by Aspergillus niger (CBS 109.713)and endo-1,4-beta-glucanase produced by Aspergillus niger (DSM 18404) having a minimum activity of:Solid form:5600 TXU and 2500 TGU/gLiquid form:5600 TXU and 2500 TGU/gCharacterisation of the active substance:endo-1,4-beta-xylanase produced by Aspergillus niger (CBS 109.713) and endo-1,4-beta-glucanase produced by Aspergillus niger (DSM 18404)Analytical methodFor quantification of endo-1,4-beta-xylanase activity:viscosimetric method based on decrease of viscosity produced by action of endo-1,4-beta-xylanase on the xylan containing substrate (wheat arabinoxylan) at pH = 3,5 and 55 °C.For quantification of endo-1,4-beta-glucanase activity:viscosimetric method based on decrease of viscosity produced by action of endo-1,4-beta-glucanase on the glucan containing substrate (barley betaglucan) at pH = 3,5 and 40 °C.Piglets(weaned)-560 TXU250 TGU-1.In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.2.For use in feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e. g. containing more than 30 % wheat, barley, rye and/or triticale.3.Recommended doses for kilogram of complete feedingstuffs:Piglets (weaned):560-840 TXU/250-375 TGU;Chickens for fattening:280-840 TXU/125-375 TGU;Laying hens:560-840 TXU/250-375 TGU;Turkeys for fattening:560-840 TXU/250-375 TGU;Ducks for fattening:280-840 TXU/125-375 TGU.4.For use in weaned piglets until approximately 35 kg.5.For safety: breathing protection, glasses and gloves shall be used during handling.22 April 2019
Chickens for fattening280 TXU125 TGU
Laying hens280 TXU125 TGU
Turkeys for fattening560 TXU250 TGU
Ducks for fattening280 TXU125 TGU
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