Commission Regulation (EC) No 333/2007 of 28 March 2007 laying down the methods of sampling and analysis for the official control of the levels of lead, cadmium, mercury, inorganic tin, 3-MCPD and benzo(a)pyrene in foodstuffs (Text with EEA relevance )
Modified by
- Commission Regulation (EU) No 836/2011of 19 August 2011amending Regulation (EC) No 333/2007 laying down the methods of sampling and analysis for the official control of the levels of lead, cadmium, mercury, inorganic tin, 3-MCPD and benzo(a)pyrene in foodstuffs(Text with EEA relevance), 32011R0836, August 20, 2011
- Commission Regulation (EU) 2016/582of 15 April 2016amending Regulation (EC) No 333/2007 as regards the analysis of inorganic arsenic, lead and polycyclic aromatic hydrocarbons and certain performance criteria for analysis(Text with EEA relevance), 32016R0582, April 16, 2016
- Commission Implementing Regulation (EU) 2019/2093of 29 November 2019amending Regulation (EC) No 333/2007 as regards the analysis of 3-monochloropropane-1,2-diol (3-MCPD) fatty acid esters, glycidyl fatty acid esters, perchlorate and acrylamide(Text with EEA relevance), 32019R2093, December 9, 2019
- Commission Implementing Regulation (EU) 2021/705of 28 April 2021amending Regulation (EC) No 333/2007 as regards the required number of incremental samples and the performance criteria for some methods of analysis(Text with EEA relevance), 32021R0705, April 29, 2021
- Commission Implementing Regulation (EU) 2022/685of 28 April 2022amending Regulation (EC) No 333/2007 as regards the sampling requirements for fish and terrestrial animals(Text with EEA relevance), 32022R0685, April 29, 2022
- Commission Implementing Regulation (EU) 2022/2418of 9 December 2022amending Regulation (EC) No 333/2007 as regards the methods for analysis for the control of the levels of trace elements and processing contaminants in foodstuffs(Text with EEA relevance), 32022R2418, December 12, 2022
≥ | 500 tonnes |
> 300 and < | 3 sublots |
≥ 100 and ≤ 300 | 100 tonnes |
< 100 | — |
≥ 15 | 15-30 tonnes |
< 15 | — |
< 50 | 3 |
≥ 50 and ≤ 500 | 5 |
> 500 | 10 |
≤ 25 | at least 1 package or unit |
26-100 | about 5 %, at least 2 packages or units |
> 100 | about 5 %, at maximum 10 packages or units |
1-50 | 1 | Entire content of the package |
51-250 | 2 | Entire content of the package |
251- | 4 | From each retail package taken for sample, half of the content of the package |
> | 4 + 1 packages per | |
Unknown (only applicable for e-commerce) | 1 | Entire content of the package |
Where the lot to be sampled contains small fish (individual fish weighing < 1 kilogram), the whole fish is taken as incremental sample to form the aggregate sample. Where the resulting aggregate sample weighs more than 3 kilograms, the incremental samples may consist of the middle parts of the fish, weighing each at least 100 grams, forming the aggregate sample. The whole part to which the maximum level is applicable, is used for homogenisation of the sample. The middle part of the fish is where the centre of gravity is. This is located in most cases at the dorsal fin (in case the fish has a dorsal fin) or halfway between the gill opening and the anus. Where the lot to be sampled contains larger fish (individual fish weighing ≥ 1 kilogram), the incremental sample consists of the middle part of the fish. Each incremental sample weighs at least 100 grams. For fish of intermediate size (≥ 1 kilogram and < 6 kilograms) the incremental sample is taken as a slice of the fish from backbone to belly in the middle part of the fish. For very large fish (≥ 6 kilograms), the incremental sample is taken from the right side (frontal view) dorso-lateral muscle meat in the middle part of the fish. Where the taking of such a piece of the middle part of the fish would result in a significant economic damage, the taking of three incremental samples of at least 350 grams each may be considered as being sufficient independent of the size of the lot or alternatively three incremental samples of at least 350 grams each from an equal part (175 grams) of the muscle meat close to the tail part and the muscle meat close to the head part of each fish may be considered as being sufficient independent of the size of the lot.
(a) Performance criteria for methods of analysis for lead, cadmium, mercury, inorganic tin and inorganic arsenic Table 5 Parameter Criterion Applicability Foods specified in Regulation (EC) No 1881/2006 Specificity Free from matrix or spectral interferences Repeatability (RSD r )HORRAT r less than 2Reproducibility (RSD R )HORRAT R less than 2Recovery The provisions of point D.1.2. apply LOD = three tenths of LOQ LOQ Inorganic tin ≤ 10 mg/kg Lead ML ≤ 0,02 mg/kg 0,02 < ML < 0,1 mg/kg ML ≥ 0,1 mg/kg ≤ ML ≤ two thirds of the ML ≤ one fifth of the ML Cadmium, mercury ML ≤ 0,02 mg/kg 0,02 < ML < 0,1 mg/kg ML is ≥ 0,1 mg/kg ≤ two fifths of the ML ≤ two fifths of the ML ≤ one fifth of the ML Inorganic arsenic and total arsenic ML ≤ 0,03 mg/kg 0,03 < ML < 0,1 mg/kg ML is ≥ 0,1 mg/kg ≤ ML ≤ two thirds of the ML ≤ two thirds of the ML (b) Performance criteria for methods of analysis for 3-monochloropropane-1,2-diol (3-MCPD), 3-MCPD fatty acid esters and glycidyl fatty acid esters: Performance criteria for methods of analysis for 3-MCPD in foods specified in point 4.1 of the Annex to Regulation (EC) No 1881/2006 Table 6a Parameter Criterion Applicability Foods specified in point 4.1 of the Annex to Regulation (EC) No 1881/2006 Specificity Free from matrix or spectral interferences Field blanks Less than LOD Repeatability (RSD r )0,66 times RSD R as derived from (modified) Horwitz equationReproducibility (RSD R )as derived from (modified) Horwitz equation Recovery 75-110 % Limit of Detection (LOD) ≤ 5 μg/kg (on dry matter basis) Limit of Quantification (LOQ) ≤ 10 μg/kg (on dry matter basis) Performance criteria for methods of analysis for 3-MCPD in foods specified in point 4.3 of the Annex to Regulation (EC) No 1881/2006 Table 6b Parameter Criterion Applicability Foods specified in point 4.3 of the Annex to Regulation (EC) No 1881/2006 Specificity Free from matrix or spectral interferences Field blanks Less than LOD Repeatability (RSD r )0,66 times RSD R as derived from (modified) Horwitz equationReproducibility (RSD R )as derived from (modified) Horwitz equation Recovery 75-110 % Limit of Detection (LOD) ≤ 7 μg/kg Limit of Quantification (LOQ) ≤ 14 μg/kg Performance criteria for methods of analysis for 3-MCPD fatty acid esters, expressed as 3-MCPD , in foods specified in point 4.3 of the Annex to Regulation (EC) No 1881/2006Table 6c Parameter Criterion Applicability Foods specified in point 4.3 of the Annex to Regulation (EC) No 1881/2006 Specificity Free from matrix or spectral interferences Repeatability (RSD r )0,66 times RSD R as derived from (modified) Horwitz equationReproducibility (RSD R )as derived from (modified) Horwitz equation Recovery 70-125 % Limit of Detection (LOD) Three tenths of LOQ Limit of Quantification (LOQ) for foods specified in 4.3.1 and 4.3.2 ≤ 100 μg/kg in oils and fats Limit of Quantification (LOQ) for foods specified in 4.3.3 and in 4.3.4 with a fat content < 40 % ≤ two fifths of the ML Limit of Quantification (LOQ) for foods specified in 4.3.4 with a fat content ≥ 40 % ≤ 15 μg/kg fat Performance criteria for methods of analysis for glycidyl fatty acid esters, expressed as glycidol, in foods specified in point 4.2 of the Annex to Regulation (EC) No 1881/2006 Table 6d Parameter Criterion Applicability Foods specified in point 4.2 of the Annex to Regulation (EC) No 1881/2006 Specificity Free from matrix or spectral interferences Repeatability (RSD r )0,66 times RSD R as derived from (modified) Horwitz equationReproducibility (RSD R )as derived from (modified) Horwitz equation Recovery 70-125 % Limit of Detection (LOD) Three tenths of LOQ Limit of Quantification (LOQ) for foods specified in 4.2.1 and 4.2.2 ≤ 100 μg/kg in oils and fats Limit of Quantification (LOQ) for foods specified in 4.2.3 with a fat content < 65 % and in 4.2.4 with a fat content < 8 % ≤ two fifths of the ML Limit of Quantification (LOQ) for foods specified in 4.2.3 with a fat content ≥ 65 % and in 4.2.4 with a fat content ≥ 8 % ≤ 31 μg/kg fat
(c) Performance criteria for methods of analysis for polycyclic aromatic hydrocarbons: The four polycyclic aromatic hydrocarbons to which these criteria apply are benzo(a)pyrene, benz(a)anthracene, benzo(b)fluoranthene and chrysene. Table 7 Parameter Criterion Applicability Foods specified in Regulation (EC) No 1881/2006 Specificity Free from matrix or spectral interferences, verification of positive detection Repeatability (RSD r )HORRAT r less than 2Reproducibility (RSD R )HORRAT R less than 2Recovery 50-120 % LOD ≤ 0,30 μg/kg for each of the four substances LOQ ≤ 0,90 μg/kg for each of the four substances (d) Performance criteria for methods of analysis for acrylamide: Table 8 Parameter Criterion Applicability All foods Specificity Free from matrix or spectral interferences Field blanks Less than Limit of Detection (LOD) Repeatability (RSD r )0,66 times RSD R as derived from (modified) Horwitz equationReproducibility (RSD R )as derived from (modified) Horwitz equation Recovery 75-110 % Limit of Detection (LOD) Three tenths of LOQ Limit of Quantification (LOQ) For foods with benchmark levels < 125 μg/kg: ≤ two fifths of the benchmark level, however not required to be lower than 20 μg/kg For foods with benchmark level ≥ 125 μg/kg: ≤ 50 μg/kg (e) Performance criteria for methods of analysis for perchlorate: Table 9 Parameter Criterion Applicability All foods Specificity Free from matrix or spectral interferences Repeatability (RSD r )0,66 times RSD R as derived from (modified) Horwitz equationReproducibility (RSD R )as derived from (modified) Horwitz equation Recovery 70-110 % Limit of Detection (LOD) Three tenths of LOQ Limit of Quantification (LOQ) ≤ two fifths of the ML (f) Notes to the performance criteria: The Horwitz equation (for concentrations 1,2 x 10W. Horwitz, L.R. Kamps, K.W. Boyer, J.Assoc.Off.Analy.Chem.,63, 1980, 1344-1354. -7 ≤ C ≤ 0,138) and the modified Horwitz equation (for concentrations C < 1,2 × 10M. Thompson, Analyst, 125, 2000, 385-386. -7 ) are generalised precision equations which are independent of analyte and matrix but solely dependent on concentration for most routine methods of analysis.Modified Horwitz equation for concentrations C < 1,2 × 10 -7 :RSD R = 22 %where: RSD R is the relative standard deviation calculated from results generated under reproducibility conditionsC is the concentration ratio (i.e. 1 = 100g/100g, 0,001 = 1000 mg/kg). The modified Horwitz equation applies to concentrations C < 1,2 × 10-7 .
Horwitz equation for concentrations 1,2 x 10 -7 ≤ C ≤ 0,138:RSD R = 2C(- 0,15 )where: RSD R is the relative standard deviation calculated from results generated under reproducibility conditionsC is the concentration ratio (i.e. 1 = 100g/100g, 0,001 = 1000 mg/kg). The Horwitz equation applies to concentrations 1,2 x 10-7 ≤ C ≤ 0,138.
Uf is the maximum standard measurement uncertainty (μg/kg). LOD is the limit of detection of the method (μg/kg). The LOD must meet the performance criteria set in point C.3.3.1 for the concentration of interest. C is the concentration of interest (μg/kg); α is a numeric factor to be used depending on the value of C. The values to be used are given in Table 10.
C (μg/kg) | α |
---|---|
≤ 50 | |
51-500 | |
501- | |
> |
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