Article 1The Annexes to Directive 93/85/EEC are hereby replaced by the corresponding texts in Annex to this Directive.

Article 21.Member States shall adopt and publish, by 31 March 2007 at the latest, the laws, regulations and administrative provisions necessary to comply with this Directive. They shall forthwith communicate to the Commission the text of those provisions and a correlation table between those provisions and the Directive.They shall apply those provisions from 1 April 2007.When Member States adopt those provisions, they shall contain a reference to this Directive or shall be accompanied by such reference on the occasion of their official publication. Member States shall determine how such reference is to be made.2.Member States shall immediately communicate to the Commission the text of the main provisions of national law which they adopt in the field covered by this Directive.

Article 3This Directive shall enter into force on the third day following that of its publication in the Official Journal of the European Union.

Article 4This Directive is addressed to the Member States.

Done at Brussels, 12 June 2006.For the CommissionMarkos KyprianouMember of the CommissionAppendix 1Laboratories involved in optimisation and validation of protocols

Contact scientists: see website http://forum.europa.eu.int/Public/irc/sanco/Home/main

Laboratory	Location	Country
Agentur für Gesundheit und Ernährungssicherheit	Vienna and Linz	Austria
Departement Gewasbescherming	Merelbeke	Belgium
Plantedirektoratet	Lyngby	Denmark
Central Science Laboratory	York	England
Scottish Agricultural Science Agency	Edinburgh	Scotland
Laboratoire National de la Protection des Végétaux, Unité de Bactériologie	Angers	France
Laboratoire National de la Protection des Végétaux, Station de Quarantaine de la Pomme de Terre	Le Rheu	France
Biologische Bundesanstalt	Kleinmachnow	Germany
Pflanzenschutzamt Hannover	Hannover	Germany
State Laboratory	Dublin	Ireland
Plantenziektenkundige Dienst	Wageningen	Netherlands
Norwegian Crop Research Institute, Plant Protection Centre	Aas	Norway
Direcção-Geral de Protecção das Culturas	Lisbon	Portugal
Nacionalni institut za biologijo	Ljubljana	Slovenia
Centro de Diagnostico de Aldearrubia	Salamanca	Spain

ANNEX ITEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF THE RING ROT BACTERIUM, CLAVIBACTER MICHIGANENSIS (Smith) Davis et al. ssp. SEPEDONICUS (Spieckermann et Kotthoff) Davis et al.SCOPE OF THE TEST SCHEMEThe presented scheme describes the various procedures involved in:(i)Diagnosis of ring rot in potato tubers and plants;(ii)Detection of Clavibacter michiganensis ssp. sepedonicus in samples of potato tubers and plants;(iii)Identification of Clavibacter michiganensis ssp. sepedonicus (C. m. subsp. sepedonicus).GENERAL PRINCIPLESOptimized protocols for the various methods, validated reagents and details for the preparation of test and control materials are provided in the Appendices. A list of the laboratories that were included in optimization and validation of protocols is provided in Appendix 1.Since the protocols involve detection of a quarantine organism and will include the use of viable cultures of C. m. subsp. sepedonicus as control materials, it will be necessary to perform the procedures under suitable quarantined conditions with adequate waste disposal facilities and under the conditions of appropriate licences as issued by the official plant quarantine authorities.Testing parameters must assure consistent and reproducible detection of levels of C. m. subsp. sepedonicus at the set thresholds of the selected methods.Precise preparation of positive controls is imperative.Testing according to the required thresholds also implies correct settings, maintenance and calibration of equipment, careful handling and preservation of reagents and all measures to prevent contamination between samples, e.g. separation of positive controls from test samples. Quality control standards must be applied to avoid administrative and other errors, especially concerning labelling and documentation.A suspected occurrence, as referred to in article 4(2) in directive 93/85/EEC implies a positive result in diagnostic or screening tests performed on a sample as specified in flow charts.If the first screening test (IF or PCR/FISH) is positive, then contamination with Cms is suspected and a second screening test must be done. If the second screening test is positive, then the suspicion is confirmed (suspected occurrence) and the testing according to the scheme must be continued. If the second screening test is negative, then the sample is considered not contaminated with Cms.Therefore a positive IF test as referred to in article 4(2) is defined by a positive IF reading confirmed by a second screening test (PCR/FISH).Confirmed presence as referred to in article 5(1) in directive 93/85/EEC implies the isolation and identification of a pure culture of C. m. subsp. sepedonicus with confirmation of pathogenicity.1.FLOW CHART DIAGRAM PRESENTATION1.1.Detection scheme for the diagnosis of Ring Rot in potato tubers and potato plants with symptoms of ring rotThe testing procedure is intended for potato tubers and plants with symptoms typical or suspect of ring rot. It involves a rapid screening test, isolation of the pathogen from infected vascular tissue on diagnostic media and, in case of a positive result, identification of the culture as C. m. subsp. sepedonicus. 32006L0056_en_img_1

1.2.Scheme for detection and identification of Clavibacter michiganensis ssp. sepedonicus in samples of asymptomatic potato tubersPrincipleThe testing procedure is intended for detection of latent infections in potato tubers. A positive result from at least two screening tests, based on different biological principles, must be complemented by the isolation of the pathogen; followed by, in case of isolation of typical colonies, confirmation of a pure culture as C. m. subsp. sepedonicus. A positive result from only one of the screening tests is not sufficient to consider the sample suspect.Screening tests and isolation tests must permit a detection threshold of 103 to 104 cells/ml resuspended pellet, included as positive controls in each series of tests. 32006L0056_en_img_2

1.3.Scheme for detection and identification of Clavibacter michiganensis ssp. sepedonicus in samples of asymptomatic potato plants

Amendments and corrections
December 31, 2021	Implicitly repealed by 32016R2031
July 7, 2006	Entry into force - Date pub. + 3 See Art 3

Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot