Commission Implementing Regulation (EU) No 304/2014 of 25 March 2014 concerning the authorisation of the preparations of Enterococcus faecium NCIMB 10415, Enterococcus faecium DSM 22502 and Pediococcus acidilactici CNCM I-3237 as feed additives for all animal species Text with EEA relevance
Modified by
  • Commission Implementing Regulation (EU) 2024/251of 16 January 2024concerning the renewal of the authorisation of the preparations of Lactiplantibacillus plantarum CNCM I-3235, Lactiplantibacillus plantarum DSM 11672/CNCM I-3736, Pediococcus acidilactici CNCM I-3237, Pediococcus acidilactici DSM 11673/CNCM I-4622, Pediococcus pentosaceus NCIMB 12455, Acidipropionibacterium acidipropionici CNCM I-4661, Lentilactobacillus buchneri NCIMB 40788/CNCM I-4323 and Lentilactobacillus hilgardii CNCM I-4785 and Lentilactobacillus buchneri CNCM I-4323/NCIMB 40788 as feed additives for all animal species, amending Implementing Regulations (EU) No 1065/2012, (EU) No 1119/2012, (EU) No 1113/2013 and (EU) No 304/2014 and repealing Implementing Regulations (EU) No 990/2012 and (EU) 2019/764(Text with EEA relevance), 32024R0251, January 17, 2024
Commission Implementing Regulation (EU) No 304/2014of 25 March 2014concerning the authorisation of the preparations of Enterococcus faecium NCIMB 10415, Enterococcus faecium DSM 22502 and Pediococcus acidilactici CNCM I-3237 as feed additives for all animal species(Text with EEA relevance)
Article 1AuthorisationThe preparations specified in the Annex belonging to the additive category "technological additives" and to the functional group "silage additives", are authorised as additives in animal nutrition, subject to the conditions laid down in that Annex.
Article 2Transitional measuresThe preparations specified in the Annex and feed containing them, which are produced and labelled before 15 October 2014 in accordance with the rules applicable before 15 April 2014 may continue to be placed on the market and used until the existing stocks are exhausted.
Article 3Entry into forceThis Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.ANNEX
Details of the analytical methods are available at the following address of the Reference Laboratory: http://irmm.jrc.ec.europa.eu/EURLs/EURL_feed_additives/Pages/index.aspxCategory of technological additives. Functional group: silage additives
Identification number of the additiveName of the holder of authorisationAdditiveComposition, chemical formula, description, analytical methodSpecies or category of animalMaximum ageMinimum contentMaximum contentOther provisionsEnd of period of authorisation
CFU/kg of fresh material
1k20601—Enterococcus faeciumNCIMB 10415Additive compositionPreparation of Enterococcus faeciumNCIMB 10415 containing a minimum of 1 × 1010 CFU/g additive.Characterisation of the active substanceViable cells of Enterococcus faeciumNCIMB 10415.Analytical methodEnumeration in the feed additive: spread plate method using Bile Esculine Azide agar (EN 15788).Identification: Pulsed Field Gel Electrophoresis (PFGE).All animal species———1.In the directions for use of the additive and premixture, indicate the storage conditions.2.Minimum content of the additive when used without combination with other micro-organisms as silage additives: 1 × 108 CFU/Kg fresh material.3.For safety: it is recommended to use breathing protection, eye protection and gloves during handling.15 April 2024
1k20602—Enterococcus faeciumDSM 22502Additive compositionPreparation of Enterococcus faeciumDSM 22502 containing a minimum of 1 × 1011 CFU/g additive.Characterisation of the active substanceViable cells of Enterococcus faeciumDSM 22502.Analytical methodEnumeration in the feed additive: spread plate method using Bile Esculine Azide agar (EN 15788).Identification: Pulsed Field Gel Electrophoresis (PFGE).All animal species1.In the directions for use of the additive and premixture, indicate the storage conditions.2.Minimum content of the additive when used without combination with other micro-organisms as silage additives: 1 × 108 CFU/Kg fresh material.3.For safety: it is recommended to use breathing protection, eye protection and gloves during handling.
1k21009—Pediococcus acidilacticiCNCM I-3237Additive compositionPreparation of Pediococcus acidilacticiCNCM I-3237 containing a minimum of 1 × 1010 CFU/g additive.Characterisation of the active substanceViable cells of Pediococcus acidilacticCNCM I-3237.Analytical methodEnumeration in the feed additive: spread plate method using MRS agar (EN 15786)Identification: Pulsed Field Gel Electrophoresis (PFGE).All animal species———1.In the directions for use of the additive and premixture, indicate the storage conditions.2.Minimum content of the additive when used without combination with other micro-organisms as silage additives: 5 × 107 CFU/Kg fresh material.3.For safety: it is recommended to use breathing protection, eye protection and gloves during handling.15 April 2024
Article 1AuthorisationThe preparations specified in the Annex belonging to the additive category "technological additives" and to the functional group "silage additives", are authorised as additives in animal nutrition, subject to the conditions laid down in that Annex.
Article 2Transitional measuresThe preparations specified in the Annex and feed containing them, which are produced and labelled before 15 October 2014 in accordance with the rules applicable before 15 April 2014 may continue to be placed on the market and used until the existing stocks are exhausted.
Article 3Entry into forceThis Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.ANNEX
Details of the analytical methods are available at the following address of the Reference Laboratory: http://irmm.jrc.ec.europa.eu/EURLs/EURL_feed_additives/Pages/index.aspxCategory of technological additives. Functional group: silage additives
Identification number of the additiveName of the holder of authorisationAdditiveComposition, chemical formula, description, analytical methodSpecies or category of animalMaximum ageMinimum contentMaximum contentOther provisionsEnd of period of authorisation
CFU/kg of fresh material
1k20601—Enterococcus faeciumNCIMB 10415Additive compositionPreparation of Enterococcus faeciumNCIMB 10415 containing a minimum of 1 × 1010 CFU/g additive.Characterisation of the active substanceViable cells of Enterococcus faeciumNCIMB 10415.Analytical methodEnumeration in the feed additive: spread plate method using Bile Esculine Azide agar (EN 15788).Identification: Pulsed Field Gel Electrophoresis (PFGE).All animal species———1.In the directions for use of the additive and premixture, indicate the storage conditions.2.Minimum content of the additive when used without combination with other micro-organisms as silage additives: 1 × 108 CFU/Kg fresh material.3.For safety: it is recommended to use breathing protection, eye protection and gloves during handling.15 April 2024
1k20602—Enterococcus faeciumDSM 22502Additive compositionPreparation of Enterococcus faeciumDSM 22502 containing a minimum of 1 × 1011 CFU/g additive.Characterisation of the active substanceViable cells of Enterococcus faeciumDSM 22502.Analytical methodEnumeration in the feed additive: spread plate method using Bile Esculine Azide agar (EN 15788).Identification: Pulsed Field Gel Electrophoresis (PFGE).All animal species1.In the directions for use of the additive and premixture, indicate the storage conditions.2.Minimum content of the additive when used without combination with other micro-organisms as silage additives: 1 × 108 CFU/Kg fresh material.3.For safety: it is recommended to use breathing protection, eye protection and gloves during handling.
1k21009—Pediococcus acidilacticiCNCM I-3237Additive compositionPreparation of Pediococcus acidilacticiCNCM I-3237 containing a minimum of 1 × 1010 CFU/g additive.Characterisation of the active substanceViable cells of Pediococcus acidilacticCNCM I-3237.Analytical methodEnumeration in the feed additive: spread plate method using MRS agar (EN 15786)Identification: Pulsed Field Gel Electrophoresis (PFGE).All animal species———1.In the directions for use of the additive and premixture, indicate the storage conditions.2.Minimum content of the additive when used without combination with other micro-organisms as silage additives: 5 × 107 CFU/Kg fresh material.3.For safety: it is recommended to use breathing protection, eye protection and gloves during handling.15 April 2024