1. Part D is replaced by the text set out in Annex I to this Regulation. 2. In Part E, the first sentence is replaced by the following: "This method of sampling is of application for the official control of the maximum levels established for ochratoxin A, aflatoxin B1 and total aflatoxins in spices." 3. Part G is replaced by the text set out in Annex II to this Regulation. 4. A Part K, as set out in Annex III to this Regulation, is added.
Commission Regulation (EU) No 178/2010 of 2 March 2010 amending Regulation (EC) No 401/2006 as regards groundnuts (peanuts), other oilseeds, tree nuts, apricot kernels, liquorice and vegetable oil (Text with EEA relevance)
| Commodity | Lot weight (tonne) | Weight or number of sublots | No incremental samples | Aggregate sample weight (kg) |
|---|---|---|---|---|
| Dried figs | ≥ 15 | 15-30 tonnes | ||
| < 15 | — | 10-100 | ≤ 30 |
On condition that the sublot can be separated physically, each lot shall be subdivided into sublots following table 1. Taking into account that the weight of the lot is not always an exact multiple of the weight of the sublots, the weight of the sublot may exceed the mentioned weight by a maximum of 20 %. Each sublot shall be sampled separately, Number of incremental samples: 100, Weight of the aggregate sample = 30 kg which shall be mixed and to be divided into three equal laboratory samples of 10 kg before grinding (this division into three laboratory samples is not necessary in case of dried figs subjected to further sorting or other physical treatment and of the availability of equipment which is able to homogenise a 30 kg sample). Each laboratory sample of 10 kg shall be separately ground finely and mixed thoroughly to achieve complete homogenisation, in accordance with the provisions laid down in Annex II, If it is not possible to carry out the method of sampling described above because of the unacceptable commercial consequences resulting from damage to the lot (because of packaging forms, means of transport, etc.) an alternative method of sampling may be applied provided that it is as representative as possible and is fully described and documented.
| Lot weight (tonnes) | No of incremental samples | Aggregate sample Weight (kg) (in case of retail packings, weight of aggregate sample can diverge — see point D.1.1) | No of laboratory samples from aggregate sample |
|---|---|---|---|
| ≤ 0,1 | 1 (no division) | ||
| > 0,1 – ≤ 0,2 | 1 (no division) | ||
| > 0,2 – ≤ 0,5 | 1 (no division) | ||
| > 0,5 – ≤ 1,0 | 9 (- < 12 kg) | 1 (no division) | |
| > 1,0 – ≤ 2,0 | |||
| > 2,0 – ≤ 5,0 | 18 (- < 24 kg) | ||
| > 5,0 – ≤ 10,0 | |||
| > 10,0 – ≤ 15,0 |
Weight of the aggregate sample ≤ 30 kg which shall be mixed and divided into two or three equal laboratory samples of ≤ 10 kg before grinding (this division into two or three laboratory samples is not necessary in case of dried figs, subjected to further sorting or other physical treatment and of the availability of equipment which is able to homogenise up to 30 kg samples). In cases where the aggregate sample weights are less than 30 kg, the aggregate sample shall be divided into laboratory samples according to following guidance: < 12 kg: no division into laboratory samples; ≥ 12 – < 24 kg: division into two laboratory samples; ≥ 24 kg: division into three laboratory samples.
Each laboratory sample shall be separately ground finely and mixed thoroughly to achieve complete homogenisation, in accordance with the provisions laid down in Annex II, If it is not possible to carry out the method of sampling described above because of the unacceptable commercial consequences resulting from damage to the lot (because of packaging forms, means of transport, etc.) an alternative method of sampling may be applied provided that it is as representative as possible and is fully described and documented.
Number of incremental samples: 100; for lots of under 50 tons the number of incremental samples shall be 10 to 100, depending on the lot weight (see table 3), Table 3 Number of incremental samples to be taken depending on the weight of the lot Lot weight (tonnes) No of incremental samples Aggregate sample weight (kg) ≤ 1 10 1 > 1 – ≤ 3 20 2 > 3 – ≤ 10 40 4 > 10 – ≤ 20 60 6 > 20 – ≤ 50 100 10 The weight of the incremental sample shall be about 100 grams. In the case of lots in retail packing, the weight of the incremental sample depends on the weight of the retail packing, Weight of aggregate sample = 1-10 kg sufficiently mixed,
acceptance if the aggregate sample or the average of the laboratory samples conforms to the maximum limit, taking into account the correction for recovery and measurement uncertainty, rejection if the aggregate sample or the average of the laboratory samples exceeds the maximum limit beyond reasonable doubt taking into account the correction for recovery and measurement uncertainty,
acceptance if none of the laboratory samples exceeds the maximum limit, taking into account the correction for recovery and measurement uncertainty, rejection if one or more of the laboratory samples exceeds the maximum limit beyond reasonable doubt taking into account the correction for recovery and measurement uncertainty,
acceptance if the laboratory sample conforms to the maximum limit, taking into account the correction for recovery and measurement uncertainty, rejection if the laboratory sample exceeds the maximum limit beyond reasonable doubt taking into account the correction for recovery and measurement uncertainty,
| Commodity | Lot weight (tonne) | Weight or number of sublots | No incremental samples | Aggregate sample weight (kg) |
|---|---|---|---|---|
| Groundnuts (peanuts), other oilseeds, apricot kernels and tree nuts | ≥ 500 | 100 tonnes | ||
| > 125 and < 500 | 5 sublots | |||
| ≥ 15 and ≤ 125 | 25 tonnes | |||
| < 15 | — | 10-100 | ≤ 20 |
On condition that the sublot can be separated physically, each lot shall be subdivided into sublots following table 1. Taking into account that the weight of the lot is not always an exact multiple of the weight of the sublots, the weight of the sublot may exceed the mentioned weight by a maximum of 20 %. Each sublot shall be sampled separately, Number of incremental samples: 100, Weight of the aggregate sample = 20 kg which shall be mixed and to be divided into two equal laboratory samples of 10 kg before grinding (this division into two laboratory samples is not necessary in case of groundnuts (peanuts), other oilseeds, apricot kernels and tree nuts subjected to further sorting or other physical treatment and of the availability of equipment which is able to homogenise a 20 kg sample). Each laboratory sample of 10 kg shall be separately ground finely and mixed thoroughly to achieve complete homogenisation, in accordance with the provisions laid down in Annex II, If it is not possible to carry out the method of sampling described above because of the commercial consequences resulting from damage to the lot (because of packaging forms, means of transport, etc.) an alternative method of sampling may be applied provided that it is as representative as possible and is fully described and documented.
| Lot weight (tonnes) | No of incremental samples | Aggregate sample Weight (kg) (in case of retail packings, weight of aggregate sample can diverge — see point D.2.1) | No of laboratory samples from aggregate sample |
|---|---|---|---|
| ≤ 0,1 | 1 (no division) | ||
| > 0,1 – ≤ 0,2 | 1 (no division) | ||
| > 0,2 – ≤ 0,5 | 1 (no division) | ||
| > 0,5 – ≤ 1,0 | 1 (no division) | ||
| > 1,0 – ≤ 2,0 | 8 (- < 12 kg) | 1 (no division) | |
| > 2,0 – ≤ 5,0 | |||
| > 5,0 – ≤ 10,0 | |||
| > 10,0 – ≤ 15,0 |
Weight of the aggregate sample ≤ 20 kg which shall be mixed and if necessary divided into two equal laboratory samples of ≤ 10 kg before grinding (this division into two laboratory samples is not necessary in case of, groundnuts (peanuts), other oilseeds, apricot kernels and tree nuts subjected to further sorting or other physical treatment and of the availability of equipment which is able to homogenise up to 20 kg samples). In cases where the aggregate sample weights are less than 20 kg, the aggregate sample shall be divided into laboratory samples according to following guidance: < 12 kg: no division into laboratory samples; ≥ 12 kg division into two laboratory samples.
Each laboratory sample shall be separately ground finely and mixed thoroughly to achieve complete homogenisation, in accordance with the provisions laid down in Annex II, If it is not possible to carry out the method of sampling described above because of the unacceptable commercial consequences resulting from damage to the lot (because of packaging forms, means of transport, etc.) an alternative method of sampling may be applied provided that it is as representative as possible and is fully described and documented.
Number of incremental samples: 100; for lots of under 50 tons the number of incremental samples shall be 10 to 100, depending on the lot weight (see table 3), Table 3 Number of incremental samples to be taken depending on the weight of the lot Lot weight (tonnes) No of incremental samples Aggregate sample weight (kg) ≤ 1 10 1 > 1 – ≤ 3 20 2 > 3 – ≤ 10 40 4 > 10 – ≤ 20 60 6 > 20 – ≤ 50 100 10 The weight of the incremental sample shall be about 100 grams. In the case of lots in retail packing, the weight of the incremental sample depends on the weight of the retail packing, Weight of aggregate sample = 1-10 kg sufficiently mixed,
acceptance if the aggregate sample or the average of the laboratory samples conforms to the maximum limit, taking into account the correction for recovery and measurement uncertainty, rejection if the aggregate sample or the average of the laboratory samples exceeds the maximum limit beyond reasonable doubt taking into account the correction for recovery and measurement uncertainty,
acceptance if none of the laboratory samples exceeds the maximum limit, taking into account the correction for recovery and measurement uncertainty, rejection if one or both of the laboratory samples exceeds the maximum limit beyond reasonable doubt taking into account the correction for recovery and measurement uncertainty,
acceptance if the laboratory sample conforms to the maximum limit, taking into account the correction for recovery and measurement uncertainty, rejection if the laboratory sample exceeds the maximum limit beyond reasonable doubt taking into account the correction for recovery and measurement uncertainty;.
----------------------
| Commodity | Lot weight (ton) | Weight or number of sublots | No incremental samples | Aggregate sample Weight (kg) |
|---|---|---|---|---|
| Roasted coffee beans, ground roasted coffee, soluble coffee, liquorice root and liquorice extract | ≥ 15 | 15-30 tonnes | ||
| < 15 | — | 10-100 | 1-10 |
On condition that the sublot can be separated physically, each lot shall be subdivided into sublots following table 1. Taking into account that the weight of the lot is not always an exact multiple of the weight of the sublots, the weight of the sublot may vary from the mentioned weight by a maximum of 20 %. Each sublot shall be sampled separately, Number of incremental samples: 100, Weight of the aggregate sample = 10 kg, If it is not possible to carry out the method of sampling described above because of the unacceptable commercial consequences resulting from damage to the lot (because of packaging forms, means of transport, etc.) an alternative method of sampling may be applied provided that it is as representative as possible and is fully described and documented.
| Lot weight (tonnes) | No of incremental samples | Aggregate sample weight (kg) |
|---|---|---|
| ≤ 0,1 | ||
| > 0,1 – ≤ 0,2 | ||
| > 0,2 – ≤ 0,5 | ||
| > 0,5 – ≤ 1,0 | ||
| > 1,0 – ≤ 2,0 | ||
| > 2,0 – ≤ 5,0 | ||
| > 5,0 – ≤ 10,0 | ||
| > 10,0 – ≤ 15,0 |
acceptance if the laboratory sample conforms to the maximum limit, taking into account the correction for recovery and measurement uncertainty, rejection if the laboratory sample exceeds the maximum limit beyond reasonable doubt taking into account the correction for recovery and measurement uncertainty.
----------------------
The weight of the incremental sample shall be at least about 100 grams (ml) (depending of the nature of the consignment e.g. vegetable oil in bulk, at least 3 incremental samples of about 350 ml have to be taken), resulting in an aggregate sample of at least 1 kg (litre), The minimum number of incremental samples to be taken from the lot shall be as given in Table 1. The lot shall be thoroughly mixed insofar possible by either manual or mechanical means immediately prior to sampling. In this case, a homogeneous distribution of aflatoxin can be assumed within a given lot, it is therefore sufficient to take three incremental samples from a lot to form the aggregate sample. Table 1 Minimum number of incremental samples to be taken from the lot On condition that the sublot can be separated physically, large bulk consignments/lots of vegetable oils shall be subdivided into sublots as foreseen in table 2 of this part. Form of commercialisation Weight of lot (in kg) Volume of lot (in litres) Minimum number of incremental samples to be taken Bulk — 3 packages ≤ 50 3 packages > 50 to 500 5 packages > 500 10 Table 2 Subdivision of lots into sublots depending on lot weight Commodity Lot weight (tonne) Weight or number of sublots Minimum N o incremental samplesMinimum aggregate sample weight (kg) Vegetable oils ≥ 1500 500 tonnes 3 1 > 300 and < 1500 3 sublots 3 1 ≥ 50 and ≤ 300 100 tonnes 3 1 < 50 — 3 1
acceptance if the laboratory sample conforms to the maximum limit, taking into account the correction for recovery and measurement uncertainty, rejection if the laboratory sample exceeds the maximum limit beyond reasonable doubt taking into account the correction for recovery and measurement uncertainty.
----------------------