| Parameters | G | I | Minimum sampling frequenc | Method of analysis and inspection |
---|
| Microbiological: | | | | |
1 | Total coliforms | /100 ml | 500 | 10000 | Fortnightly(1) | Fermentation in multiple tubes. Subculturing of the positive tubes on a confirmation medium. Count according to MPN (most probable number) or membrane filtration and culture on an appropriate medium such as Tergitol lactose agar, endo agar, 0,4 % Teepol broth, subculturing and identification of the suspect colonies.In the case of 1 and 2, the incubation temperature is variable according to whether total or faecal coliforms are being investigated. |
2 | Faecal coliforms | /100 ml | 100 | 2000 | Fortnightly(1) |
3 | Faecal streptococci | /100 ml | 100 | — | (2) | Litsky method. Count according to MPN (most probable number) or filtration on membrane. Culture on an appropriate medium.Fermentation in multiple tubes. Subculturing of the positive tubes on a confirmation medium. Count according to MPN (most probable number) or membrane filtration and culture on an appropriate medium such as Tergitol lactose agar, endo agar, 0,4 % Teepol broth, subculturing and identification of the suspect colonies. |
4 | Salomonella | /1 l | — | 0 | (2) | Concentration by membrane filtration. Inoculation on a standard medium. Enrichment — subculturing on isolating agar — identification. |
5 | Entero viruses | PFU/10 l | — | 0 | (2) | Concentrating by filtration, flocculation or centrifuging and confirmation. |
| Physico-chemical: | | | | |
6 | pH | | — | 6 to 9 (0) | (2) | Electrometry with calibration at pH 7 and 9 |
7 | Colour | | — | No abnormal change in colour(0) | Fortnightly(1) | Visual inspection or photometry with standards on the Pt.Co scale. |
| | | — | — | (2) | |
8 | Mineral oils | mg/l | — | No film visible on the surface of the water and no odour | Fortnightly(1) | Visual and olfactory inspection or extraction using an adequate volume and weighing the dry residue. |
| | | 0,3 | — | (2) |
9 | Surface-activesubstancesreacting withmethylene blue | mg/l(lauryl-sulfate) | — | No lasting foam | Fortnightly(1) | Visual inspection or absorption spectrophotometry with methylene blue. |
| | | ± 0,3 | — | (2) | |
10 | Phenols(phenol indices) | mg/lC5H5OH | — | No specific odour | Fortnightly(1) | Verification of the absence of specific odour due to phenol or absorption spectrophotometry 4-aminoantipyrine (4 AAP) method. |
| | | ± 0,005 | ± 0,005 | (2) |
11 | Transparency | m | 2 | 1 (0) | Fortnightly(1) | Secchi's disc. |
12 | Dissolved oxygen | % saturation O2 | 80 to 120 | — | 2 | Winkler's method or electrometric method (oxygen meter). |
13 | Tarry residues and floating materials such as wood, plastic articles, bottles, containers of glass, plastic, rubber or any other substance. waste or splinters. | | Asence | | Fortnightly(1) | Visual inspection. |
14 | Ammonia | mg/l NH4 | | | (3) | Absorption spectrophotometry, Nessler's method, or indophenol blue method. |
15 | Nitrogen Kjeldahl | mg/l N | | | (3) | Kjeldahl method. |
| Other substances regarded as indications of pollution | | | | |
16 | Pesticides(parathlon, HCH, diedrin) | mg/l | | | (2) | Extraction with appropriate solvents and chromatographic determination |
17 | Heavy metals such as: | | | | | Atomic absorption possibly preceded by extraction |
| —arsenic | mg/l As | | | (2) |
| —cadmiuim | Cd | | | |
| —chrome VI | Cr VI | | | |
| —lead | Pb | | | |
| —mercury | Hg | | | |
18 | Cyanides | mg/l Cn | | | (2) | Absorption spectrophotometry using a specific reagent |
19 | Nitrates and | mg/l NO3 | | | (3) | Absorption spectrophotometry using a specific reagent |
| Phosphates | PO4 | | | |